Use of White Light Fluorescence LED in long-term Live Cell Imaging Applications

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The use of fluorescence light microscopy for live cell imaging has increased dramatically since the discovery of fluorescence proteins1  (Tsien et al.). Currently, we are able to fluorescently tag most proteins in order to study their localization and dynamic behavior. Despite these developments, long-term live-cell imaging can still be challenging as extensive light exposure might lead to cytotoxic stress.